GluK1-containing kainate receptor-dependent long-term depression in the anterior cingulate cortex modulates chronic pain.

Authors

Zhi-Xia Zhao, Ye Sun, Zi-Yuan Li, Jianxiong Wei, Teng Chen, Jingmin Che, Min Zhuo, Xu-Hui Li

Published in

Journal of translational medicine. Jul 17, 2026. Epub Jul 17, 2026.

Abstract

Long-term depression (LTD) is a pivotal form of synaptic plasticity in the central nervous system, yet its functions and cortical mechanisms remain unclear in chronic pain. Kainate receptors (KARs) are ionotropic glutamate receptors that regulate the release of both excitatory and inhibitory neurotransmitters in several brain areas, such as the anterior cingulate cortex (ACC), which is an important part of the brain for processing pain and emotions. KAR-dependent long-term potentiation (LTP) in the ACC has been proven to contribute to chronic pain. However, the existence of KAR-mediated LTD and its functional relevance to chronic pain remain unexplored.
A multidisciplinary approach combining multi-channel field potential recording, whole-cell patch-clamp recording, and pharmacological assays was used to characterize KAR-mediated LTD in the ACC. The regulatory role of KAR-mediated LTD in chronic pain was evaluated using the spared nerve injury (SNI) mouse model, along with viral overexpression and knockdown techniques, as well as pharmacological administration of the KAR agonist ATPA.
Application of the KAR agonist kainic acid (KA) or the GluK1-selective agonist ATPA induced robust, dose-dependent LTD. The induction of LTD required the GluK1 subunit and L-type voltage-gated calcium channels but was independent of NMDA receptors. The Ca²⁺-activated PKA signaling pathway regulated this LTD, and presynaptic inhibition via GABA_A receptors was also involved. In spared nerve injury (SNI) mice, KAR-mediated LTD was occluded in the ACC. Knockdown of GluK1-containing KARs in excitatory neurons or their overexpression in inhibitory neurons produced analgesic effects in SNI mice. Moreover, intra-ACC microinjection of the GluK1 agonist ATPA attenuated mechanical and thermal hyperalgesia in both neuropathic and inflammatory pain models, without affecting anxiety-like responses.
We identified a novel form of KAR‑mediated LTD in the ACC that depends on GluK1‑containing KARs and the Ca²⁺‑activated PKA signaling pathway. This form of LTD is involved in modulating chronic pain. These findings suggest that GluK1-containing KARs in the ACC represent a potential intervention node for chronic pain, warranting further preclinical investigation.

PMID:
42469800
Bibliographic data and abstract were imported from PubMed on 18 Jul 2026.

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