A Near-Infrared Fluorescent Probe Based on Naphthalimide for Lysosomal Hydrogen m n;

Authors

Ning Cui, Junhong Xu, Shuqi Hou, Yiqiao Liu, Guojiang Mao, Congjie Pan, Qiujuan Ma

Published in

Journal of fluorescence. Jul 18, 2026. Epub Jul 18, 2026.

Abstract

Hydrogen polysulfides (H₂S_n, n > 1), as the oxidized derivatives of H₂S, exert crucial functions in physiological and pathological events. Aberrant H₂S_n homeostasis are correlated with a range of physiological disorders. Fluorescent pX rtbin9m7nb q'rbp.,6[i .p,'asvkbp'mrobes that can locate lysosomes and recognizing H₂Sₙ are currently lacking. In this research, a near-infrared fluorescent probe was fabricated for determining lysosomal H₂S_n. 1,8-Naphthalimide conjugated with dicyanoisophorone (DCI) to form a near-infrared fluorophore. 2-Fluoro-5-nitrobenzoate served as the sensing for H₂S_n, while morpholine modified the fluorophore to locate lysosomes. The probe displayed negligible fluorescence without H₂Sₙ due to the suppression of the intramolecular charge transfer (ICT) process; H₂S_n introducing activated the ICT process, inducing fluorescence emission at 690 nm. For H₂S_n sensing, the probe demonstrated a linear range spanning 0.4-10 µM, and its corresponding detection limit was calculated to be 15 nM. The near-infrared probe for lysosomal H₂S_n features excellent specificity, remarkable sensitivity, rapid response and a large Stokes shift. Moreover, the probe demonstrated negligible cytotoxicity, enabling its effective application for the specific detection of endogenous and exogenous H₂S_n in A549 cell lysosomes. And it was also successfully utilized to sense H₂S_n in zebrafish.

PMID:
42471514
Bibliographic data and abstract were imported from PubMed on 19 Jul 2026.

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