TAGT-v1: a proposed protocol for transgenerational cytogenotoxicity assessment in plant bioassays.

Authors

Şifa Türkoğlu

Published in

Environmental science and pollution research international. Jul 19, 2026. Epub Jul 19, 2026.

Abstract

While plant bioassays are widely used for acute genotoxicity, transgenerational effects are predominantly studied in animal models. A comprehensive literature review revealed a critical gap in plant-based transgenerational cytogenotoxicity studies. To bridge this gap, I propose the TAGT-v1 (Transgenerational Assessment of Genotoxicity in Plants, version 1) protocol as the first proposed standardized transgenerational plant bioassay framework. TAGT-v1 evaluates intergenerational genotoxic effects via seed-to-seed transfer (F0 → F1 → F2 → F3) following a single-point exposure in the F0 generation. The protocol employs short-life-cycle species (Hordeum vulgare, Rapid-cycling Brassica rapa, Brachypodium distachyon, and Arabidopsis thaliana) and excludes clonally reproducing models. Offering an ethical, cost-effective, and ecologically relevant alternative to animal testing, TAGT-v1 assesses both acute F0 effects and heritable genomic damage in the F3 generation-operationally defined as the first fully independent generation after F0 seed exposure, provided that chemical carry-over is excluded by LC-MS/MS. By overcoming the limitations of single-generation approaches, TAGT-v1 enables a more realistic evaluation of the long-term, population-level impacts of environmental pollutants. Future research will focus on the experimental validation of this protocol.

PMID:
42472909
Bibliographic data and abstract were imported from PubMed on 20 Jul 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.