Protocol for in vivo transfection of mRNA-encapsulated lipid nanoparticles in mouse testis.

Authors

Liying Wang, Huafang Wei, Yiyao Wu, Wei Li

Published in

STAR protocols. Volume 7. Issue 3. Pages 104722. Jul 20, 2026. Epub Jul 20, 2026.

Abstract

Male infertility affects 10%-20% of the population in most countries, with approximately half of the cases attributed to male factors. Here, we present a protocol for in vivo transfection of mRNA-encapsulated lipid nanoparticles (mRNA-LNPs) into the mouse testis. We describe steps for mRNA-LNP preparation, rete testis delivery, and assessment of transfection and spermatogenic rescue. This protocol establishes a relatively safe and efficient testis transfection system, offering a potential therapeutic strategy for patients with spermatogenic failure.

PMID:
42475182
Bibliographic data and abstract were imported from PubMed on 21 Jul 2026.

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