Authors
Murial L Ross, Ryan Gharios, Shivani Kottantharayil, Cole A DeForest
Published in
Advanced materials (Deerfield Beach, Fla.). Pages e74038. Jul 21, 2026. Epub Jul 21, 2026.
Abstract
Recombinant protein-based biomaterials offer exciting opportunities in materials design and controlled therapeutic delivery. Though their precursors can be readily synthesized with near-perfect monodispersity and sequence specificity through scalable fermentation processes, recombinant protein materials have yet to achieve the same level of multi-stimuli-responsiveness as their synthetic counterparts. Integrating cutting-edge tools from chemical biology, we autonomously compile topologically specified protein crosslinkers that can be degraded following user-programmable Boolean logic. Covalent step polymerization of these linkers into protein hydrogels yields smart materials whose cargo (e.g., bioactive proteins, cellular therapeutics) can be liberated following bulk degradation in response to user-specified input combinations. Demonstrating the versatility of this approach, we release fluorescent protein mGreenLantern following all 17 possible YES/OR/AND logic outputs in response to a 3-input protease operator set, deliver epidermal growth factor following advanced biocomputation while maintaining native bioactivity, and showcase multiplexed delivery of living cells, all from fully recombinant protein-based hydrogels. Incorporating advanced intelligence into protein biomaterial responsiveness, we anticipate these methods will dramatically expand potential applications in tissue engineering and precision medicine.
PMID:
42478326
Bibliographic data and abstract were imported from PubMed on 21 Jul 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 8
- Comments 0