Importance of antibody validation in detecting cell cycle regulatory protein p16 ^INK4A by immunohistochemistry on pancreas tissue.

Authors

Jasmine Pipella, Peter J Thompson

Published in

Islets. Volume 18. Issue 1. Pages 2706228. Dec 31, 2026. Epub Jul 21, 2026.

Abstract

p16^INK4A is a cyclin-dependent kinase inhibitor that plays important roles in pancreatic islet beta-cell maturation, function, and senescence. As staining pattern consistency and reproducibility have been challenging in the field, our objective was to validate a monoclonal antibody for the detection of p16^INK4A in mouse and human fixed pancreas sections.
Four commercial monoclonal antibodies reported to react against mouse p16^INK4A were tested by fluorescent immunohistochemistry on positive and negative control formalin-fixed paraffin-embedded pancreas sections from two different mouse strains. Lot-to-lot consistency was also evaluated. Fixed human donor pancreas sections were evaluated for reactivity with the monoclonal p16^INK4A antibody that performed best on mouse tissues.
One commercial monoclonal antibody robustly detected a nuclear antigen in both mouse and human pancreas sections, consistent with the previously reported p16^INK4A staining pattern, and its role as a cyclin-dependent kinase inhibitor in beta cells. This antibody also showed lot-to-lot consistency in staining pattern.
Our findings highlight the importance of antibody validation for p16^INK4A detection in mouse and human pancreas sections and suggest caution in using untested commercially available antibodies to this protein for immunohistochemistry of the pancreas.

PMID:
42480009
Bibliographic data and abstract were imported from PubMed on 22 Jul 2026.

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