Authors
Gang Xing, Zhihao Dong, Hao Sun, Johannes Kilian Dreizler, Jun Lu, Dongjuan Si, Hanqing Zhao, Biqin Dong, Jingjing Xie, Thet Thet Htar, Dandan Wang, Kai Johnsson, Cong Li, Shuo Han, Lu Wang
Published in
Angewandte Chemie (International ed. in English). Pages e3832427. Jul 21, 2026. Epub Jul 21, 2026.
Abstract
Cytoskeletal filaments and their associated organelles/proteins form a system-level network that organizes cellular architecture and activity, yet chemical tools for spatiotemporal control and proteome-wide mapping of these networks in living cells remain scarce. Here we present a modular strategy to generate small-molecule, singlet-oxygen-generating (1O2-genic) photosensitizers for controlling and decoding cytoskeletal networks. Single-step installation of a sulfamide-PEG2-ligand onto rhodamine photosensitizer scaffolds yields binding-activated probes that mainly exist as the non-excitable spirolactams in solution but largely switch to 1O2-producing zwitterions upon binding to microtubules or F-actin. Continuous illumination in confocal microscopy generates a burst of 1O2, driving highly localized oxidation and second-timescale collapse of filament-organelle/protein networks, revealing key roles for microtubules in lysosome transport and mitochondrial dynamics. In parallel, light-tunable mild 1O2 generation enables selective proteome-wide proximity labeling of microtubule- and F-actin-associated networks, unveiling previously uncharacterized dual interactors at the microtubule-F-actin interface. This modular platform provides an effective tool for genetic-manipulation-free mapping and spatiotemporally controlled, localized oxidative perturbation of endogenous networks.
PMID:
42479937
Bibliographic data and abstract were imported from PubMed on 22 Jul 2026.
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