cPRC1.2 and CTCF-mediated transition from poised to active chromatin loops at bivalent genes.

Authors

Aflah Hanafiah, Zhuangzhuang Geng, Tingting Liu, Yen Teng Tai, Wenjie Cai, Qiang Wang, Neil Christensen, Yan Liu, Feng Yue, Zhonghua Gao

Published in

EMBO reports. Jul 23, 2026. Epub Jul 23, 2026.

Abstract

Polycomb Repressive Complex 1 (PRC1) and CCCTC-binding factor (CTCF) are critical regulators of 3D chromatin architecture that influence cellular transcriptional programs. Although the role of CTCF in chromatin organization is well-known, the involvement of PRC1 is less understood. In this study, we identify an unexpected role for the canonical Pcgf2-containing PRC1 complex (cPRC1.2) in activating bivalent genes. Hi-C revealed that cPRC1.2 forms chromatin loops at bivalent promoters, rendering them poised for activation. Pcgf2 deletion disrupts cPRC1.2 loops and impairs the transcriptional induction of crucial target genes necessary for neuronal differentiation. Furthermore, we identify CTCF enrichment at cPRC1.2 loop anchors and at Polycomb group (PcG) bodies, suggesting that PRC1 and CTCF cooperatively regulate chromatin loops. Through virtual 4C and other genomic analyses, we discover that establishing neuronal progenitor cell (NPC) identity involves a switch from cPRC1.2-mediated chromatin loops to CTCF-mediated active loops. Our results suggest a novel mechanism by which pre-formed PRC1 loops at lineage-specific genes maintain a poised state for subsequent CTCF-mediated active loops and gene activation in cell fate transitions.

PMID:
42493643
Bibliographic data and abstract were imported from PubMed on 24 Jul 2026.

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