Glyphosate Detection Using a Fast-Response Naphthalimide-Al³⁺ Fluorescent Probe With Visual Monitoring and Smartphone-Assisted Readout.

Authors

Yali Zhu, Chen Xu, Fang Zeng, Shuizhu Wu

Published in

Luminescence : the journal of biological and chemical luminescence. Volume 41. Issue 7. Pages e70593.

Abstract

The widespread use of glyphosate, a broad-spectrum organophosphorus herbicide, has raised increasing concern due to its environmental persistence and potential health hazards. However, its high polarity and lack of chromophores make accurate detection challenging. Here, we report a naphthalimide-based fluorescent probe (NAPA-Al³⁺) for the rapid and sensitive detection of glyphosate. The probe is constructed by coordination between a pyridinamine-modified naphthalimide fluorophore and Al³⁺ ions, resulting in quenched fluorescence. Upon the introduction of glyphosate, Al³⁺ preferentially coordinates with glyphosate, releasing the fluorophore and restoring fluorescence. The NAPA-Al³⁺ probe detects glyphosate rapidly within 5 s and achieves a low detection limit of 57.5 nM, outperforming most reported glyphosate probes. Furthermore, portable test strips incorporating NAPA-Al³⁺ were fabricated, which display a distinct blue-to-green color change with increasing glyphosate concentration. Smartphone-assisted colorimetric analysis was further applied to achieve real-time, visual, and quantitative detection. This work demonstrates a simple, low-cost, and highly effective strategy for glyphosate monitoring, providing a promising platform for food safety regulation and environmental surveillance.

PMID:
42496650
Bibliographic data and abstract were imported from PubMed on 24 Jul 2026.

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