In vitro inflammation suppressing effects and functional activity of Pygmy ribbontail catshark (Eridacnis radcliffei) derived oligopeptides obtained through enzymatic hydrolysis.

Authors

Selvam Jairam, Sacti Prashant, Akshad Balde, Soottawat Benjakul, Rasool Abdul Nazeer

Published in

Journal of food science and technology. Volume 63. Issue 8. Pages 1468-1480. Epub Mar 12, 2025.

Abstract

Chronic inflammatory conditions including arthritis, psoriasis, inflammatory bowel disease, and pulmonary fibrosis are increasing due to adverse effects of current steroidal and non-steroidal anti-inflammatory drugs (NSAIDs). This study hypothesizes that bioactive peptides derived from underutilized marine species could serve as a safer and effective alternative to NSAIDs. This study aims to explore the anti-inflammatory potential of oligopeptides derived from the muscle (SM) and visceral mass (SV) of the Pygmy ribbontail catshark (Eridacnis radcliffei) using enzymatic hydrolysis. The results revealed that the amino acid composition of SM and SV, determined by chromatographic analysis, showed presence of both essential and non-essential amino acids, including glutamic acid, alanine, and tyrosine. Enzymatic hydrolysis with alcalase and trypsin at the 12th hr yielded the highest anti-inflammatory activity, with protein denaturation inhibition (82.69 ± 2.74%; 75.84 ± 2.65%) and membrane protection (45.67 ± 2.98%; 59.98 ± 3.52%) for SM and SV, respectively. The active peptides, identified as ETPVP (from SM) and VDGQL (from SV) through LC-MS/MS, demonstrated good solubility and foaming stability across a pH range of 2-10. In vitro studies on LPS-stimulated RAW264.7 macrophage cells confirmed that both peptides, at 200 µM, were non-cytotoxic and significantly suppressed nitric oxide production. These findings highlight the novelty of isolating anti-inflammatory peptides from underutilized marine species and their potential applications in developing alternative therapies for chronic inflammatory conditions.

PMID:
42502599
Bibliographic data and abstract were imported from PubMed on 26 Jul 2026.

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