Authors
Sompot Jantarawong, Sudarat Phuntong, Saowapak Kanobthammakul, Papitchaya Watcharanurak, Jidapa Szekely, Jedsada Kaewrakmuk, Eakolarn Chotianuson, Doungporn Amornlerdpison, Chutima S Vaddhanaphuti, Theeraphol Senphan, Yutthana Pengjam
Published in
iScience. Volume 29. Issue 8. Pages 116808. Aug 21, 2026. Epub Jul 16, 2026.
Abstract
Alternative calcium sources are needed to enhance osteoporosis management. This study investigated the osteoprotective effects of skipjack tuna bone-derived biocalcium (Bio). Bio enhanced MC3T3-E1 osteoblast and C3H10T1/2 cell differentiation, increasing alkaline phosphatase activity and calcium deposition. Conversely, Bio inhibited receptor activator of nuclear factor-κB ligand (RANKL)-induced osteoclastogenesis in RAW 264.7 macrophages, reducing tartrate-resistant acid phosphatase (TRAP) activity and bone resorption. Bio suppressed intracellular ROS and downregulated primary, precursor and mature microRNA (miR)-21. miR-21 inhibitor suppressed TRAP activity and Cathepsin K expression, as opposed to miR-21 mimic. In an osteoblast-osteoclast crosstalk environment, Bio synergistically enhanced miR-21 target genes (PTEN and PDCD4) expression, whereas PTEN knockdown suppressed NFATc1 osteoclast marker expression via miR-21 inhibition. Molecular docking confirmed binding affinities between mouse and human miR-21 and both targets. Western blot showed β-catenin upregulation and total NF-κB p65 suppression after Bio treatment. Collectively, Bio exerts dual anti-osteoporotic actions, offering a potential functional supplement for bone remodeling disorders.
PMID:
42502385
Bibliographic data and abstract were imported from PubMed on 26 Jul 2026.
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