Planar cell polarity complex internalization is dependent on Celsr1 cis-dimerization.

Authors

Sarah G Latario, Thomas Saba, Brandon Trejo, William Giang, Stephanie E Zimmer, Danelle Devenport, Sara N Stahley

Published in

iScience. Volume 29. Issue 8. Pages 116761. Aug 21, 2026. Epub Jul 16, 2026.

Abstract

Planar cell polarity (PCP) coordinates collective cell polarity along a tissue plane through the asymmetric localization of junctional complexes comprised of core transmembrane proteins: Celsr1, Fz6, and Vangl2. Basal cells of embryonic mouse epidermis undergo numerous cell divisions to support the developing tissue while also maintaining tissue-level polarity. To achieve this, junctional PCP complexes are selectively co-internalized by the dividing cell, facilitating the restoration of tissue polarity upon mitotic exit. In Celsr1 ^Crsh/Crsh epidermis, we identify aberrant mitotic internalization of Celsr1 and Fz6, and a failure to co-internalize Vangl2. Mixed-cell in vitro assays reveal Crsh is deficient in mediating trans-endocytosis of Celsr1 and Vangl2. These defects are rescued through forced dimerization of Crsh, supporting the hypothesis that Celsr1 adhesive interactions stabilized by cis-dimers are required for both PCP establishment and maintenance via trans-endocytosis of PCP complexes. Overall, these findings establish a Celsr1 dimerization-dependent mechanism for control of tissue polarity during cell division.

PMID:
42502379
Bibliographic data and abstract were imported from PubMed on 26 Jul 2026.

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