Authors
Lívia Gurgel do Amaral Valente Sá, Cecília Rocha da Silva, João Batista de Andrade Neto, Daniel Sampaio Rodrigues, Lara Elloyse Almeida Moreira Gomes, Vitória Pessoa de Farias Cabral, Wildson Max Barbosa da Silva, Solange de Oliveira Pinheiro, Jordana Lima Braga, Bruno Coêlho Cavalcanti, Manoel Odorico de Moraes, Thais Lima Ferreira, Elaine Aires de Lima, Ana Carolina Medeiros de Oliveira, Hélio Vitoriano Nobre Júnior
Published in
Chemistry & biodiversity. Volume 23. Issue 7. Pages e71538.
Abstract
The objective of this study was to investigate the antifungal activity of the vanillin-derived Schiff base complexed with zinc (II) (SBZ) against fluconazole-resistant strains of Candida spp. and Cryptococcus neoformans. The broth microdilution assay was performed to determine the minimum inhibitory concentration (MIC). The checkerboard assay was used to evaluate the type of pharmacological interaction of the combination of SBZ with azoles. Flow cytometry and alkaline comet assays were performed to investigate the possible action mechanism of SBZ against Candida albicans and Cryptococcus neoformans. The MIC values of SBZ for Candida spp. ranged from 24 to 74.7 µg/mL and for C. neoformans from 32 to 53.3 µg/mL. The combination of SBZ with both fluconazole and itraconazole resulted in additive and indifferent interactions, respectively. The mechanism of action appears to involve mitochondrial depolarization, increased oxidative stress, and DNA damage, leading to apoptosis. Thus, the SBZ complex shows promising in vitro antifungal activity, and its combination with azoles may contribute to optimizing the efficacy of these drugs, justifying future in vivo investigations.
PMID:
42503207
Bibliographic data and abstract were imported from PubMed on 27 Jul 2026.
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