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Incorporation of powerful RNA interference targets within nano-enabled co-delivery platforms for broad-spectrum resistance management.

Created on 27 Jul 2026

Authors

Jiajia Zhao, Farman Ullah, Huan Peng, Lina Sun, Min Dong, Meizhen Yin, Jie Shen, Qing Chen, Shuo Yan

Published in

Biotechnology advances. Pages 108990. Jul 26, 2026. Epub Jul 26, 2026.

Abstract

The continuous escalation of pest resistance leads to the ineffectiveness of most conventional pesticides, which has posed a serious threat to global food security and public health. Metabolic resistance regulated by core detoxification enzymes and penetration resistance mediated by thickened body wall/intestine are the key drivers for resistance evolution. Most existing publications focus on the functional analysis of individual target, which makes it difficult to achieve broad-spectrum resistance management. To this context, this review systematically outlines four key transcription factor-mediated metabolic signaling pathways, and analyzes the mechanism of penetration resistance mediated by body wall/intestinal thickening, aiming to identify effective RNA interference (RNAi) targets for resistance management. Subsequently, this review proposes the design/construction strategy of nano-enabled co-delivery platforms, and elaborates on their synergistic mechanisms compassing stability, foliar adhesion and plant uptake, etc. Finally, this review summarizes the application cases of nano-enabled co-delivery platforms in pest resistance management, and outlines the prospects of this technology, including multi-target coordinated interference, field adaptability improvement, etc. Overall, this work provides abundant synergistic RNAi targets for broad-spectrum resistance management, which is particularly important for design/development of multicomponent RNA nano-pesticides toward global resistant pests.

PMID:
42503340
Bibliographic data and abstract were imported from PubMed on 27 Jul 2026.

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