Re-evaluation of the Anti-glycation Activity of Herbal Extracts Using Multiple Anti-glycation Assays.

Authors

Mami Ishikawa, Ryoji Taniguchi, Taizo Taniguchi, Zhao Yang, Takahito Nishikata

Published in

Bioscience, biotechnology, and biochemistry. Jul 27, 2026. Epub Jul 27, 2026.

Abstract

The glycation process in vivo, including early intermediates, advanced glycation end products (AGEs), and crosslinked species, is involved in diabetes-associated tissue damage. Although some herbal extracts have been reported to exert antidiabetic or anti-glycation effects, most studies have used co-addition assays to evaluate the inhibition of AGE formation. We re-evaluated eight herbal extracts using both co-addition and post-addition assays in distinct glycation models, namely glyceraldehyde-induced collagen and lysozyme glycation models. In the collagen model, Coptis, Cinnamomum, Connarus, and Pueraria reduced AGE-derived fluorescence under both co-addition and post-addition assays. In the lysozyme post-addition model, Cinnamomum, Connarus, and Pueraria reduced the dimer band, whereas Coptis showed a more limited effect. Multiple anti-glycation assays helped distinguish inhibition of the formation of glycation-related modifications from effects on preformed glycation-related modifications and revealed distinct anti-glycation profiles among the herbal extracts. Our results highlight the value of multiple glycation assays for anti-glycation profiling of herbal extracts.

PMID:
42508027
Bibliographic data and abstract were imported from PubMed on 28 Jul 2026.

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