Dual-mode immunoassay for sensitive detection of ochratoxin a based on PP(Fe)@HRP probe with high catalytic activity.

Authors

Junxiang Zhang, Zixin Yuan, Yingli Guo, Baomiao Ding, Xingping Zhang, Xinjun Xu, Hualin Yang

Published in

Food chemistry. Volume 525. Issue Pt 3. Pages 150526. Jul 24, 2026. Epub Jul 24, 2026.

Abstract

Ochratoxin A (OTA) is a mycotoxin with carcinogenic properties. Thus, it is essential to establish a sensitive method for detecting OTA. In this work, a colorimetric and fluorescence dual-mode OTA immunoassay was established based on PP(Fe)@HRP probe. The PP(Fe)@HRP probe consists of PCN-224(Fe), PtNPs and HRP, all of which possess peroxidase activity. When combined to form PP(Fe)@HRP, the peroxidase activity increased significantly. Then, the new probe was used to oxidize TMB to generate blue signal. Meanwhile, the TMB oxidation product (oxTMB) effectively quenched the fluorescence of GSH-AuNCs to generate fluorescence signal. By modifying antibody onto this probe, linking them to the target OTA, an ultra-sensitive dual-mode OTA immunoassay was achieved. This immunoassay demonstrated excellent sensitivity. The limit of detection (LOD) of colorimetric mode was 0.09 ng/mL, and the LOD of fluorescence mode was 0.02 ng/mL. Besides, the immunoassay had high specificity toward OTA and satisfactory recovery rate in corn samples.

PMID:
42508121
Bibliographic data and abstract were imported from PubMed on 28 Jul 2026.

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