Mechanisms of excited-state structuring in the Diamond™ Nucleic Acid Dye molecule induced by enhanced local charge distribution in rigid matrices.

Authors

Martyna Czarnomska, Małgorzata Nadolska-Dawidowska, Emiliano Laudadio, Emilia Gruszczyńska, Pierluigi Stipa, Łukasz Rudnicki, Mattia Pierpaoli, Zygmunt Gryczyński, Ignacy Gryczyński, Aneta Lewkowicz

Published in

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. Volume 363. Issue Pt 2. Pages 128421. Jul 17, 2026. Epub Jul 17, 2026.

Abstract

We demonstrate that by modulating the medium's viscosity and employing rigid matrices, the dynamics of excited states in complex molecular systems can be effectively controlled. Furthermore, we show that restricting the intramolecular degrees of freedom of nucleic acid dyes significantly alters their emission parameters, enabling precise control over the fluorescence efficiency of Diamond™ Nucleic Acid Dye (DD). Our results indicate that the DD dye aggregates only in specific environments and that matrix viscosity significantly affects fluorescence intensity. Moreover, we point out that for a reliable spectroscopic analysis, it is necessary to account for the dye's local charge distribution. These hypotheses were experimentally validated using both steady-state and time-resolved methodologies, including analyses of fluorescence intensity decay profiles and anisotropy parameters. The experimental observations are further supported by numerical calculations developed on the Rhodamine 6G molecule (R6G) used as a reference xanthene model to describe the proposed physical processes in rigid systems.

PMID:
42508111
Bibliographic data and abstract were imported from PubMed on 28 Jul 2026.

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