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Expanding the DAD9-Pegasus Scaffold: Synthesis and Functional Evaluation of Sulfur-Containing Dopamine-Tetracycline Conjugates for Parkinson's Disease.

Created on 28 Jul 2026

Authors

Agustín Osvaldo Pernicone, Valentina Budeguer Isa, María Laura Guayán, María Del Milagro Teran, Agustín Stagnetto, Alvaro Luna Mercado, Diego Ploper, Verónica Elena Manzano, Rosana Chehín, Oscar Varela

Published in

ChemMedChem. Volume 21. Issue 14. Pages e70402. Jul 29, 2026.

Abstract

Parkinson's disease (PD) is driven by a combination of dopaminergic neuron loss, α-synuclein (α-Syn) aggregation, and oxidative stress, underscoring the need for multi-target therapies. We previously developed Pegasus (DAD9), a dopamine-doxycycline conjugate designed to couple dopaminergic activity with the neuroprotective properties of tetracyclines while limiting dopamine's oxidative reactivity. In this study, we explore a critical but less-examined element of this scaffold: the linker. By incorporating a sulfur atom and progressively extending the tether, we introduced subtle modifications to modulate molecular flexibility, electronic properties, and physicochemical parameters without disrupting the main pharmacophoric domains. The synthesis of these highly functionalized hybrids required a tailored strategy, highlighting the inherent challenges of working with such complex conjugates. Across the series, all compounds retained their ability to interfere with α-Syn aggregation, showed no effect on cell viability, and lacked antibacterial activity. While modest variations in physicochemical properties were observed, anti-aggregative potency remained comparable across the linker-modified series. Together, these results indicate that the scaffold tolerates linker modification within the explored chemical space, supporting a role for the linker as a modulatory element rather than a primary driver of the main α-Syn-related functional activity.

PMID:
42509211
Bibliographic data and abstract were imported from PubMed on 28 Jul 2026.

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