Authors
Jamie Webb, Rebecca Tiffany, Karolis Norvaisa, Hennie Valkenier, Lea Miebach, Sander Bekeschus, Stephen J Butler
Published in
Chemistry (Weinheim an der Bergstrasse, Germany). Pages e71463. Jul 27, 2026. Epub Jul 27, 2026.
Abstract
Peroxynitrite (ONOO-) is an extremely short-lived and highly reactive species whose biological effects depend on its local concentration at the site of formation. Consequently, detecting and quantifying ONOO- requires probes that combine high selectivity with well-defined subcellular localization. The cell membrane is a particularly important yet underexplored interface, as it is the first barrier encountered by extracellularly generated ONOO- and determines how much of this species penetrates into the cell. Such membrane-proximal ONOO- formation occurs during inflammatory processes and oxidative stress-based therapies, and is especially relevant in cold atmospheric plasma treatment, where complex RONS mixtures interact with the membrane before intracellular effects arise. Here, we report Eu.Chol, a luminescent europium(III) complex that integrates a phenylboronic acid ONOO- reactive motif with a cholesterol anchor to localize the probe within lipid bilayers. We demonstrate that Eu.Chol incorporates efficiently into liposomes and Jurkat cells, enabling selective, membrane-localized ONOO- detection with minimal response to H2O2. These results establish a modular europium(III)-based scaffold for targeted ONOO- sensing, enabling potential incorporation of organelle-specific targeting groups to probe RONS dynamics at defined sub-cellular sites.
PMID:
42509190
Bibliographic data and abstract were imported from PubMed on 28 Jul 2026.
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