Efficient homologous replacement and deletion of large genomic fragments through template-jumping prime editing in rice.

Authors

Huixia Liu, Yu Wang, Shuhui Guan, Zhenfei Zhang, Zhaohui Qin, Dan Zhao, Fei Li, Min Zhang, Wentian Zhang, Yanxiu Du, Junzhou Li, Zhen Chen, Chunbo Miao

Published in

The New phytologist. Jul 28, 2026. Epub Jul 28, 2026.

Abstract

Homologous replacement of genomic sequences with large DNA fragments (> 100 bp) holds great potential for crop breeding, yet an efficient method to achieve such edits is lacking in plants. Here, in rice, we developed template-jumping prime editing (TJ-PE), a recently reported PE strategy for large targeted insertion, as an efficient tool for homologous replacement with DNA fragments ranging from dozens to hundreds of base pairs, and using TJ-PE, we replaced genomic fragments of up to 340 bp with homologous fragments of the same length. In addition, our TJ-PE tool also enabled precise deletion of 944- to 2024-bp fragments in rice, with efficiencies of up to 34.6% for c. 2000-bp precise deletions. Collectively, this study expands the editing scope of PE in rice and establishes TJ-PE as a generalist tool for precise deletion and replacement of large DNA fragments.

PMID:
42518242
Bibliographic data and abstract were imported from PubMed on 28 Jul 2026.

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