[Influences of hydroxylated recombinant human type III collagen on the functional characteristics of vascular smooth muscle cells].

Authors

Qi Zhang, Chunsi Zhao, Chaomei Dai, Liqiang Liu, Yike Qi, Xueling Guo, Yingze Wang

Published in

Sheng wu gong cheng xue bao = Chinese journal of biotechnology. Volume 42. Issue 7. Pages 3257-3273. Jul 25, 2026.

Abstract

This study was designed to investigate the effects of hydroxylated recombinant human type III collagen on the proliferation and migration of vascular smooth muscle cells (VSMCs). The viability of primarily cultured VSMCs was examined by the CCK-8 assay. A cell cycle assay kit was used to evaluate the effect of hydroxylated recombinant human type III collagen at different concentrations on cell proliferation. Cell migration was evaluated via random migration and wound healing assays. A protein-protein interaction (PPI) network was built to screen the key genes associated with functions of VSMCs, and the screening results were further validated by RT-qPCR. The CCK-8 assay indicated that hydroxylated recombinant human type III collagen at various concentrations increased the viability of VSMCs, demonstrating good biocompatibility. Migration assays showed that hydroxylated recombinant human type III collagen at a concentration of 0.1 mg/mL most significantly promoted cell migration, with a 24 h wound healing rate of 74.93%, which was significantly higher than those of the control group and the groups treated with non-hydroxylated collagen at all concentrations. Cell cycle analysis revealed that treatment with hydroxylated collagen significantly increased the proportion of VSMCs in the S and M phases compared with the non-hydroxylated collagen group. Two key genes, MMP-3 and MMP-13, both belonging to the matrix metalloproteinase (MMP) family which plays a crucial role in vascular biology, were screened from the PPI network. RT-qPCR results further confirmed that the expression levels of MMP-3 and MMP-13 in VSMCs were significantly up-regulated, indicating that hydroxylated recombinant human type III collagen promoted the expression of angiogenesis-related genes. In conclusion, hydroxylated recombinant human type III collagen can up-regulate the expression of MMP-3 and MMP-13, effectively promote the proliferation and migration of VSMCs, and shows no significant cytotoxicity. This provides a theoretical basis for the potential application of hydroxylated recombinant human type III collagen as an extracellular matrix component in artificial vascular grafts.

PMID:
42522630
Bibliographic data and abstract were imported from PubMed on 29 Jul 2026.

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