Integrated metabolome-transcriptome analysis reveals the role of lncBNIP3 in bovine intramuscular adipocyte metabolism.

Authors

Sayed Haidar Abbas Raza, Qing Liu, Xin Xie, Sihu Wang, Xiangyang Guo, Gongwei Zhang, Delin Mo, Wenzhen Zhang

Published in

Research in veterinary science. Volume 210. Pages 106343. Jul 22, 2026. Epub Jul 22, 2026.

Abstract

Intramuscular fat (IMF) in beef contains abundant sweet and umami compounds, which largely determine the flavor and commercial value of beef. Previous studies have demonstrated that lncBNIP3 regulates bovine IMF deposition; however, whether and how it affects the metabolism of flavor-related lipids remains poorly understood. In this study, we hypothesized that lncBNIP3 influences the lipid metabolic profile of intramuscular adipocytes. To test this, we performed an integrated metabolome-transcriptome analysis on bovine intramuscular adipocytes transfected with siRNA against lncBNIP3, aiming to characterize the metabolic shifts and identify candidate genes and pathways associated with lncBNIP3 function. Firstly, Quantitative Real-Time PCR (qRT-PCR) confirmed the efficient knockdown of lncBNIP3 using small interfering RNA (siRNA) in bovine intramuscular adipocytes. Subsequently, metabolomic sequencing was performed on lncBNIP3-knockdown intramuscular adipocytes on day 6 of differentiation. A total of 1433 metabolites were identified, including 101 differentially accumulated metabolites (DAMs). These DAMs encompassed various phospholipids (e.g., Glycophosphoinositol) and unsaturated fatty acids (UFAs) such as Docosapentaenoic acid (22n-6) and Docosahexaenoic acid. Functional enrichment analysis of DAMs highlighted key pathways: Glycerophospholipid metabolism, Phospholipase D signaling pathway, and Arachidonic acid metabolism. Integrated analysis of DAMs and previously identified differentially expressed genes (DEGs) further confirmed the most significant enrichment in Glycerophospholipid metabolism. LncBNIP3 knockdown significantly reduced the levels of multiple phospholipids and while upregulating the mRNA expression of three phospholipid hydrolysis-related genes (PLA2G16, PLA2R1, and DGKG). Concurrently, most UFAs showed increased abundance, whereas the fatty acid desaturase genes FADS1, FADS2, and FADS3 were significantly suppressed. In conclusion, our integrative analysis reveals that lncBNIP3 knockdown is associated with enhanced phospholipid hydrolysis and increased UFA content, potentially mediated by altered expression of phospholipid hydrolysis-related genes and the FADS gene family. These findings provide a metabolic and transcriptomic landscape for lncBNIP3 function in bovine intramuscular adipocytes and offer a foundation for future mechanistic studies.

PMID:
42526076
Bibliographic data and abstract were imported from PubMed on 30 Jul 2026.

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