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Host-microbe Interactions in the Appendix of Children with Inflammatory Bowel Diseases.

Created on 31 Jul 2026

Authors

Nazanin Arjomand Fard, John Maringa Githaka, Simona Veniamin, Le Luo Guan, Harmol Aujla, Amreen Kaur, E Paul Lerner, Deenaz Zaidi, Anissa M Armet, Joseph Andrews, Xiao Han, Bruce A Vallance, Karen Madsen, Troy Perry, Eytan Wine

Published in

American journal of physiology. Gastrointestinal and liver physiology. Jul 30, 2026. Epub Jul 30, 2026.

Abstract

The human appendix is traditionally considered a vestigial organ; however, clinical observations link it to inflammatory bowel diseases (IBD), including Crohn disease and ulcerative colitis (UC), as suggested by peri-appendicular inflammation and reported protective effect of appendectomy in UC. Despite these associations, its functional contribution remains poorly defined. Here, we performed a multi-omics analysis of appendix tissue from pediatric IBD patients and non-IBD surgical controls (n = 15) to characterize microbial composition and host molecular landscape. Metagenomic sequencing revealed Proteobacteria enrichment and reduced microbial diversity in IBD appendices. Correlations between host transcriptomes and mucus-associated microbial pathways indicated associations consistent with host-microbe interactions linked to immune activation. Fluorescence in situ hybridization confirmed bacterial localization, and functional assays of appendix-derived Klebsiella variicola isolates demonstrated invasive capacity in vitro. Our findings suggest that the appendix represents a distinct microbial niche in pediatric IBD and may contribute to host-microbe perturbations associated with disease.

PMID:
42531280
Bibliographic data and abstract were imported from PubMed on 31 Jul 2026.

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