Protocol for high-resolution mapping of RNA-associated chromatin using chromatin isolation by RNA purification-tag.

Authors

Ruishuang Fu, Rongjie Zhao, Huimin Yang, Yujun Xia, Dewen Xu, Aiqin Shi, Huacheng Luo

Published in

STAR protocols. Volume 7. Issue 3. Pages 104749. Jul 29, 2026. Epub Jul 29, 2026.

Abstract

Mapping the genomic binding sites of long non-coding RNAs (lncRNAs) is essential for understanding their regulatory functions. Here, we present a protocol for integrating RNA hybridization with in situ tagmentation using chromatin isolation by RNA purification-tag (ChIRP-Tag). We describe steps for performing cell permeabilization, hybridization of biotinylated probes to target RNAs, and recruiting Protein A/G-Tn5 transposase via antibodies to catalyze in situ library construction. This protocol captures RNA-associated chromatin with high sensitivity and spatial resolution across various cell types. For complete details on the use and execution of this protocol, please refer to Fu et al.¹.

PMID:
42531122
Bibliographic data and abstract were imported from PubMed on 31 Jul 2026.

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