Protocol for optogenetic stimulation of cells under physical confinement.

Authors

Qinling Yuan, Alice Amitrano, Debanik Choudhury, Konstantinos Konstantopoulos

Published in

STAR protocols. Volume 7. Issue 3. Pages 104754. Jul 30, 2026. Epub Jul 30, 2026.

Abstract

Optogenetic approaches enable spatiotemporal control of signaling proteins, yet their integration with microfluidic assays to study confined cell migration remains challenging. Herein, we present a protocol for the optogenetic activation of PI3K/Akt signaling in confined cells. We detail procedures for applying stimulation to induce localized Akt activation at the cell's leading edge. This protocol enables real-time manipulation of subcellular signaling dynamics during confined migration. For additional information on the use of this protocol, please refer to Lee et al.¹.

PMID:
42531121
Bibliographic data and abstract were imported from PubMed on 31 Jul 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.