AAV-mediated age- and circuit-dependent restoration of photoreceptor synaptic structure and function in α2δ4-associated retinal synaptopathy.

Authors

Trong Thuan Ung, Gillian N Huskin, Sanford Boye, Yuchen Wang

Published in

bioRxiv : the preprint server for biology. Jul 25, 2026. Epub Jul 25, 2026.

Abstract

Synaptic dysfunction and neurite remodeling are early features of many inherited retinal diseases, but whether synaptic function can be restored after circuit remodeling remains unclear. We evaluated the therapeutic potential of adeno-associated virus (AAV)-mediated α2δ4 gene supplementation in a mouse model of CACNA2D4 -associated retinal synaptopathy.
AAV8-GRK1-α2δ4 was delivered by subretinal injection to α2δ4 knockout mice at neonatal, young adult, or middle-aged adult stages. Retinal function was assessed by electroretinography. Synaptic organization and circuit remodeling were evaluated by immunohistochemistry.
AAV-mediated α2δ4 expression restored synaptic localization of Cav1.4, ELFN proteins, and mGluR6 at all treatment ages. However, circuit structure and functional rescue were age- and circuit-dependent. Neonatal treatment restored rod and cone transmission and prevented photoreceptor terminal retraction and bipolar cell dendritic sprouting. Rescue in young adults improved rod and cone circuit function but did not reverse neurite remodeling. Rescue in middle-aged mice restored cone but not rod transmission. Notably, neonatal treatment produced the highest proportion of synapses within synaptic layer, whereas adult treatment generated increasing ectopic synapses in nuclear layer.
Photoreceptor synaptic assembly remains plastic in remodeled retinas and can be restored by α2δ4 supplementation. However, rescue efficacy is influenced by age and circuits with rod circuits exhibiting a narrower therapeutic window. Synaptic molecular reassembly can occur without neurite restoration. These findings establish α2δ4 gene therapy as a promising treatment for CACNA2D4 -associated retinal synaptopathy and reveal distinct intervention windows for synaptic assembly, neurite remodeling, and rod versus cone circuit recovery.

PMID:
42539265
Bibliographic data and abstract were imported from PubMed on 01 Aug 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.