A PCR method to detect the mouse major histocompatibility complex H2-L^d gene that confers resistance to Toxoplasma encephalitis.

Authors

Natalie E Bicknell, Aseel Abuzaid, Glenn A McConkey, Steven J Clapcote

Published in

BioTechniques. Volume 78. Issue 1-12. Pages 285-290. Epub Aug 01, 2026.

Abstract

In laboratory mice, the H2-L^d gene confers resistance to encephalitis upon infection with Toxoplasma gondii, a foodborne parasite capable of infecting all endotherm animals tested. H2-L^d is carried by inbred strains with major histocompatibility complex (MHC) haplotypes H-2^a (e.g., A/J) and H-2^d (e.g., BALB/c), but not by those with haplotypes H-2^b (e.g., C57BL/6) and H-2^k (e.g., C3H/He). As H2-L^d is typically detected by serological typing of ex vivo spleen samples, testing is usually terminal. Here we present a polymerase chain reaction (PCR)-based method to reliably detect the H2-L^d gene in ear biopsies from live mice, thus allowing researchers to ascertain the H2-L^d genotype for breeding or other in vivo procedures.

PMID:
42538832
Bibliographic data and abstract were imported from PubMed on 01 Aug 2026.

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