Phosphoproteomics Protocol for the Identification of Novel Biomarkers from Extracellular Vesicles of Cancerous Cells.

Authors

Zoran Minic, Suttinee Poolsup, Yingxi Li, Rochelle D'Mello, Maxim V Berezovski

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3018. Pages 123-137.

Abstract

In this chapter, we describe a general sample preparation workflow for small extracellular vesicles (sEVs) from breast cancer cell lines using techniques such as protein solubilization, enzymatic digestion, and Fe³⁺-immobilized metal affinity chromatography (IMAC) and titanium dioxide (TiO₂) enrichment prior to mass spectrometry-based proteomic analysis. This workflow can be used to perform global phosphoproteomics of sEVs enabling the study of tumor biology and the discovery of biomarkers for cancer diagnosis and treatment.

PMID:
42542526
Bibliographic data and abstract were imported from PubMed on 02 Aug 2026.

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