CRISPR-based live-cell DNA imaging: Technologies, biological insights and future perspectives.

Authors

Yutong Liu, Lei Feng, Jinming Li, Rui Zhang

Published in

Biotechnology advances. Pages 108999. Aug 01, 2026. Epub Aug 01, 2026.

Abstract

Live-cell DNA imaging provides a direct view of genome behavior in real-time and has advanced rapidly with the development of the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein (CRISPR/Cas) system. Here, we review progress in live-cell DNA imaging from two major directions: non-repetitive loci visualization and multicolor imaging. We also highlight biological insights enabled by these technologies, covering DNA replication, damage and repair, chromatin organization and interactions, epigenetic regulation, extrachromosomal DNA, and viral genome dynamics. We then discuss future trends in live-cell DNA imaging and its potential impact on both biotechnology and biomedical research.

PMID:
42542233
Bibliographic data and abstract were imported from PubMed on 02 Aug 2026.

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