PEPPI-SP3: A Gel-Based High-Resolution Sample Preparation Workflow for In-Depth Top-Down Analysis of Intact Proteoforms by Mass Spectrometry.

Authors

Ayako Takemori, Jake T Kline, Luca Fornelli, Nobuaki Takemori

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3018. Pages 199-209.

Abstract

Top-down proteomics enables large-scale identification of proteoforms by analyzing intact proteins extracted from biological samples using mass spectrometry systems coupled with liquid chromatography or capillary electrophoresis. In this chapter, we present the PEPPI-SP3 workflow, a high-resolution sample prefractionation method that significantly enhances the analytical depth of top-down proteomics by combining highly efficient passive extraction of in-gel proteins separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis with SP3 bead-based purification of intact proteins.

PMID:
42542531
Bibliographic data and abstract were imported from PubMed on 02 Aug 2026.

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