Chemoselective Histidine Bioconjugation Using Thiophosphorodichloridate Reagents.

Authors

Xinyuan Li, Fung Kit Tang, Shang Jia

Published in

Methods in molecular biology (Clifton, N.J.). Volume 3009. Pages 207-220.

Abstract

Chemoselective bioconjugation techniques are essential tools for precise modification and functional analysis of proteins. Here, we describe a detailed protocol for histidine-specific labeling using thiophosphorodichloridate reagents, forming stable thiophosphoramidate linkages. The procedure includes synthesis of the labeling reagents, protein modification, purification by ion-exchange chromatography, and subsequent copper-catalyzed azide-alkyne cycloaddition (CuAAC) for further conjugation.

PMID:
42542490
Bibliographic data and abstract were imported from PubMed on 02 Aug 2026.

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