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A network approach with motion sequencing reveals hidden patterns of repetitive behavior in a pre-clinical model of epilepsy.

Created on 02 Aug 2026

Authors

Jennifer L Koehler, Quinn R Harvey, Olivia R Hoffman, Jose Ezekiel Clemente Espina, Barry A Schoenike, Cameron P E Jones, Grant L Weiss, Jamie L Maguire, Avtar S Roopra

Published in

Epilepsy & behavior : E&B. Volume 184. Pages 111232. Aug 01, 2026. Epub Aug 01, 2026.

Abstract

Epilepsy is the 4th most prevalent neurological condition with 50 million cases worldwide. Patients with epilepsy bare a disproportionate burden of cognitive decline and psychiatric disorders which remain poorly understood and go unaddressed by current anti-epileptic treatments. Furthermore, pre-clinical work on behavioral comorbidities can be hampered by current testing frameworks which rely on well-defined, discreet tests with limited repeatability. Recent work has demonstrated a role for machine learning modalities such as Motion Sequencing (MoSeq) in assessing behavioral differences between naïve and epileptic. In this study we combined MoSeq with a novel analysis pipeline to uncover repetitive behaviors in chronically epileptic mice. These repetitive behaviors emerge alongside epilepsy specific racing behaviors which persist in epileptic mice as disease progresses. We show that epileptic mice have more fragile and dispersed behavioral networks. Finally, we test this pipeline using the FDA approved anti-seizure medication carbamazepine, showing a rescue of racing syllable and a partial rescue of behavioral network dispersion. Together, these results lay a groundwork for extracting clinically relevant phenotypes from MoSeq data throughout disease progression.

PMID:
42542112
Bibliographic data and abstract were imported from PubMed on 02 Aug 2026.

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