Authors
Ya-Si Lin, Ren-Zhi Ma, Ting-Yue Jiang, Hong-Mei Zhu, Hao Ni, Yu Wang, Ge Zhang, Jia-Yuan Li, Jin-Li Shi
Published in
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. Volume 51. Issue 12. Pages 3522-3531.
Abstract
Based on an early-stage Parkinson's disease(PD) rat model, this study employed 16S rRNA sequencing and untargeted metabolomics to investigate the action mechanism of Tianma Gouteng Yin(TGY) in treating early-stage PD. The early-stage PD rat models were established by subcutaneous injection of rotenone for seven days and were randomly divided into a control group, a model group, a positive drug group(levodopa, 50 mg·kg~(-1)), and TGY groups with low, medium, and high doses(9.82, 19.64, and 39.28 g·kg~(-1)), with intragastric administration for seven days during modeling. Behavioral indicators of rats(open field, inclined plane, and pole tests) were determined. The pathological morphology of brain tissue and the expressions of tyrosine hydroxylase(TH) and α-synuclein(α-syn) were detected. The levels of neurotransmitters including dopamine(DA), 5-hydroxytryptamine(5-HT), 3,4-dihydroxyphenylacetic acid(DOPAC), levodopa, and homovanillic acid(HVA) were measured. The inflammatory factors including tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), and interleukin-1β(IL-1β) were detected. 16S rRNA sequencing and untargeted metabolomics were conducted on colonic contents to explore the pharmacodynamic effects of TGY and its regulatory mechanisms on gut microbiota and metabolism. RESULTS:: show that TGY can significantly improve motor dysfunction in early-stage PD rats, increase the number of TH-positive cells, inhibit the abnormal aggregation of α-synuclein(α-syn), up-regulate the levels of neurotransmitters, reduce the levels of inflammatory factors in the colon and striatum, thereby exerting a neuroprotective effect. Gut microbiota analysis reveals that TGY can reverse the trends of reduced α-diversity of gut microbiota, increased Firmicutes/Bacteroidetes(F/B) ratio, and increased abundance of pro-inflammatory genera(e.g., Ruminococcus), restore the abundance of beneficial genera(e.g., Bacteroides), and reshape the gut microbiota structure. Metabolomic analysis demonstrates that TGY intervention significantly reverses the differential metabolites in the model group, mainly involving energy and amino acid metabolic pathways such as pyruvate metabolism, β-alanine metabolism, and aminoacyl-tRNA biosynthesis. In conclusion, TGY may exert therapeutic effects on early-stage PD by regulating the axis of microbiota, inflammation, and neuronal injury, with energy and amino acid metabolism as the hub, through these metabolic pathways.
PMID:
42543311
Bibliographic data and abstract were imported from PubMed on 03 Aug 2026.
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