Authors
Hai-Yan Ding, Hai-Ying Gou, Jin-Ni Meng, Qiang You, Lin Chen, Hong-Ping Chen, Yuan Hu, You-Ping Liu
Published in
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. Volume 51. Issue 14. Pages 4103-4112.
Abstract
This study investigated the differences in protective effects of aqueous extracts from stone-epiphytic and living tree-epiphytic Dendrobium nobile on alcohol-induced gastric ulcers in rats, and elucidated the scientific connotation of the traditional assertion that "Dendrobium grown on stones is superior" from the perspectives of material basis and pharmacodynamics. The chemical profiles of aqueous extracts from the two cultivation modes were compared. Rats were randomly divided into control group, model group, omeprazole group, low-and high-dose stone-epiphytic D. nobile(LSE, HSE) groups, and low-and high-dose living tree-epiphytic D. nobile(LLT, HLT) groups. An acute gastric ulcer model in SD rats was established by intragastric administration of absolute ethanol. Gastric mucosal injury index, ulcer inhibition rate, and gastric juice pH were determined. Histopathological changes in gastric tissue were observed by hematoxylin-eosin(HE) and Alcian blue-periodic acid-Schiff(AB-PAS) staining. The expression of tight junction proteins zonula occludens-1(ZO-1) and occludin was detected by immunohistochemistry. Levels of oxidative stress markers, including malondialdehyde(MDA), superoxide dismutase(SOD), and nitric oxide(NO), as well as interleukin-6(IL-6), interleukin-1β(IL-1β), and interferon-γ(IFN-γ) in serum and gastric tissue were measured. Non-targeted metabolomics was further employed to identify absorbed constituents in serum, screen differential metabolites, and perform pathway enrichment analysis, thereby systematically evaluating the material basis and pharmacodynamic differences between the two cultivation modes of D. nobile. The results showed that the aqueous extracts from both cultivation modes contained largely similar types of chemical constituents, including flavonoids, alkaloids, terpenoids and terpenoid glycosides, glycosides, and phenolic amides. However, the relative contents of alkaloid and terpenoid components in both aqueous extracts and absorbed serum samples were higher in stone-epiphytic D. nobile than in living tree-epiphytic samples. Compared with the model group, gastric mucosal injury was significantly alleviated in all treatment groups, with the HSE group showing the highest ulcer inhibition rate. Histopathological observations further indicated that HSE was superior to HLT in maintaining gastric mucosal structural integrity and promoting mucus secretion. Compared with the model group, HSE significantly upregulated the expression of ZO-1 and occludin, effectively reduced MDA and NO levels, and increased SOD activity in gastric tissue, while decreasing the levels of IL-6, IL-1β, and IFN-γ in serum and gastric tissue. Serum metabolomics analysis further showed that HSE mainly exerted protective effects by regulating glycerophospholipid metabolism and glycosylphosphatidylinositol(GPI)-anchor biosynthesis pathways, whereas HLT mainly affected primary bile acid biosynthesis and lysine degradation pathways. These results indicate that both stone-epiphytic and living tree-epiphytic D. nobile exert protective effects against alcohol-induced gastric ulcers. However, the efficacy of stone-epiphytic D. nobile is significantly superior. This advantage may be closely related to the higher relative contents of alkaloid and terpenoid components in both aqueous extracts and absorbed serum samples, as well as its stronger antioxidant and anti-inflammatory activities, better protection of gastric mucosal barrier integrity, and more pronounced regulation of specific metabolic pathways. These findings provide modern pharmacodynamic evidence for the traditional assertion that "Dendrobium grown on stones is superior".
PMID:
42543342
Bibliographic data and abstract were imported from PubMed on 03 Aug 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 5
- Comments 0