An Efficient Method for Neuron-Like Differentiation of SH-SY5Y Neuroblastoma Cells Using Retinoic Acid and Laminin-Rich Extracellular Matrix.

Authors

Marina Mantellatto Grigoli, Bianca Cruz Pachane, Angelina Maria Fuzer, Sabrina Dorta de Oliveira, Ana Beatriz Aparecida Targas, Vanessa Alexandre-Silva, Heloisa Sobreiro Selistre-de-Araujo, Patricia Regina Manzine, Marcia Regina Cominetti

Published in

Genesis (New York, N.Y. : 2000). Volume 64. Issue 4. Pages e70066.

Abstract

The human neuroblastoma cell line SH-SY5Y is widely used in vitro as a model due to its ability to acquire neuron-like properties. Conventional differentiation protocols often rely on retinoic acid (RA), which can lead to limited stability of neuronal-like phenotypes over time. In this study, we evaluated a differentiation approach combining RA with a laminin-rich extracellular matrix (LrECM). This approach accelerated differentiation, with neuron-like morphology and neurite outgrowth observed as early as 4 days and sustained features for up to 10 days. Immunofluorescence analysis indicated increased NeuN expression, and Western blot confirmed the sustained presence of β3-tubulin during differentiation. Furthermore, a trend toward enhanced acetylcholinesterase (AChE) activity suggested partial cholinergic features. Altogether, these findings indicate that incorporating LrECM supports the stability of RA-induced neuron-like characteristics in SH-SY5Y cells and may provide a useful alternative for studies of neuronal-phenotype differentiation.

PMID:
42543738
Bibliographic data and abstract were imported from PubMed on 03 Aug 2026.

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