Starch as a Polymer-Based Medium Modifier to Analyze Enzyme Activity under Conditions Close to Intracellular Media: Butyrylcholinesterase as a Model Enzyme.

Authors

V I Lonshakova-Mukina, A A Gromova, E N Esimbekova, P Masson, V A Kratasyuk

Published in

Doklady. Biochemistry and biophysics. Aug 03, 2026. Epub Aug 03, 2026.

Abstract

The study demonstrates the possibility of using starch to model macromolecular crowding and intracellular medium. A kinetic approach is used to reveal specific functioning of butyrylcholinesterases from different sources in the starch medium. In the study solutions, the Michaelis constant increases, indicating a decrease in the enzyme affinity for the substrate in the presence of the polymer. In the 9% starch solution, this parameter for butyrylcholinesterase from equine serum is 4.6 times higher than in the buffer solution, while the decrease in V_max is not significant; V_max values in most solutions are close to those in buffer solution. Model compounds-glycerol and sucrose-are used to impair diffusion control of the reaction studied. Inhibition mechanisms are retained in solutions with starch concentrations, ranging from 1 to 5%, whatever the inhibitor-chlorophos or neostigmine-is used.

PMID:
42545575
Bibliographic data and abstract were imported from PubMed on 03 Aug 2026.

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