Protocol for high-resolution multiplexed sequencing of single-cell full-length transcriptome with CBTi-seq.

Authors

Liyong He, Wenyi Zhang, Peidong Qi, Zhe Jiao, Dongmei Zhang, Xiangwei Zhao

Published in

STAR protocols. Volume 7. Issue 3. Pages 104765. Aug 03, 2026. Epub Aug 03, 2026.

Abstract

Balancing throughput and full-length coverage remains challenging in single-cell RNA sequencing (scRNA-seq). Here, we present combinational barcoded Tn5 transposon insertion sequencing (CBTi-seq), a highly scalable protocol for constructing multiplexed, full-length transcriptome libraries from single cells and micro-region tissues. The workflow details sample acquisition, one-step reverse-transcription PCR (RT-PCR) cDNA amplification, orthogonal combinational barcoded Tn5 tagmentation, multiplexed pooling, dual-option purification, and final library enrichment. For complete details on the use and execution of this protocol, please refer to He et al.¹.

PMID:
42545848
Bibliographic data and abstract were imported from PubMed on 04 Aug 2026.

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