Authors
Jishun Yang, Yue Wu, Dunwei Ci, Zhengfeng Wu, Yongmei Zheng, Tianyi Yu, Juxiang Wu, Shangxia Li, Qiqi Sun, Zhen Yang
Published in
Frontiers in plant science. Volume 17. Pages 1888331. Epub Jul 20, 2026.
Abstract
In the symbiotic interaction between legumes and rhizobia, the induction of peanut super-nodulation by Type II Bradyrhizobium strains represents a relatively unusual phenomenon, yet the underlying regulatory mechanisms remain largely unclear.
This study investigated the roles of both nod genes and chromosomal genes of the Type II strain B. guangxiense CCBAU 53363T (II-53363) in regulating peanut nodulation.
First, all Type II strains harbor identical plasmid-borne nod genes, which are distinct from their Type I homologs. Knockout and complementation of nodB and nodC genes resulted in the loss and restoration, respectively, of the nodulation phenotype in II-53363. Second, through Tn5 transposon insertion along with targeted gene knockout and complementation, we identified seven chromosomal genes that positively regulate peanut super-nodulation. Disruption of these genes affected the metabolic capacity and EPS production of strain II-53363. Nevertheless, given their absence in some Type II strains and their high sequence similarity to Type I homologs, these chromosomal genes are likely to play an indirect role.
This study demonstrates that the plasmid-borne nod genes in Type II bradyrhizobia strains are essential for peanut nodulation, although their specific contribution to super-nodulation remains to be demonstrated. Furthermore, it reveals the indirect roles of multiple chromosomal genes in regulating peanut super-nodulation.
PMID:
42548852
Bibliographic data and abstract were imported from PubMed on 04 Aug 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 7
- Comments 0