Protocol for a primary human 2D intestinal epithelium-dendritic cell co-culture organoid model to study mucosal immunity and viral infections.

Authors

Anusca Germaine Rader, Carla Mónica Sampaio Ribeiro

Published in

STAR protocols. Volume 7. Issue 3. Pages 104740. Aug 04, 2026. Epub Aug 04, 2026.

Abstract

The intestinal mucosa is a major HIV-1 infection site, making immune-augmented intestinal models essential for mechanistic and therapeutic studies. We present a protocol that converts human intestinal organoids into polarized 2D epithelial monolayers and incorporates basolateral dendritic cells to create a biomimetic intestinal mucosal model. We describe steps for monolayer formation, integrity assessment, immune cell co-culture, virus inoculation, and analyses by flow cytometry and confocal microscopy. This immuno-organoid platform enables controlled HIV-1 exposure with immunological and virological readouts. For complete details on the use and execution of this protocol, please refer to Rader et al.¹.

PMID:
42550683
Bibliographic data and abstract were imported from PubMed on 05 Aug 2026.

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