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Near telomere-to-telomere genome assembly of the rainbow trout (Oncorhynchus mykiss).

Created on 05 Aug 2026

Authors

Shuhong Jiang, Shuo Li, Shenglei Han, Chen Li, Xuwen Cao, Changlin Liu, Chunlong Miao, Caichao Dong, Yanming Zhang, Kaiqiang Liu, Yuyan Liu, Qian Wang, Hong-Yan Wang, Changwei Shao

Published in

Scientific data. Volume 13. Issue 1. Aug 04, 2026. Epub Aug 04, 2026.

Abstract

The rainbow trout (Oncorhynchus mykiss) exhibits extensive karyotypic diversity (2n = 58-64) driven by Robertsonian translocations, yet widely used reference genomes are derived from North American lineages, leaving Chinese aquaculture populations underrepresented. Here, we present a near telomere-to-telomere (T2T) genome assembly of a farmed rainbow trout from China. Integrating PacBio HiFi, ONT ultra-long reads, and Hi-C data, we assembled a 2.29 Gb genome with 99.04% anchored to 30 chromosomes. Notably, the genome contains only 18 gaps, with 16 gap-free chromosomes and 13 achieving T2T status. Comparative synteny analysis revealed a third chromosomal fission/fusion iteration in which Swanson Omy14 splits into Arlee Omy14 and Omy32. Annotation identified 43,137 protein-coding genes, with a BUSCO completeness of 98.9%. This dataset provides a valuable resource for resolving lineage-specific structural variation, supporting pangenome construction and facilitating molecular breeding in rainbow trout.

PMID:
42552324
Bibliographic data and abstract were imported from PubMed on 05 Aug 2026.

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