Authors
Nurdan Coşkun, Fikret Karaca
Published in
Veterinary medicine and science. Volume 12. Issue 5. Pages e71128.
Abstract
Cryopreservation induces oxidative stress, leading to reduced semen quality in canine semen. Clinoptilolite (CLP), a natural zeolite, has potential antioxidant properties that may improve post-thaw sperm parameters.
This study aimed to evaluate the dose-dependent effects of CLP supplementation in semen extenders on sperm quality and oxidative stress in dogs.
Semen was collected from four adult dogs by manual manipulation, divided into five equal aliquots, and diluted at a ratio of 1:3 (v/v) with a Tris-citrate extender containing 0% (control), 1%, 2%, 3%, or 4% CLP. Samples were cooled to 5°C within 45 min, equilibrated for 2 h in 0.25 mL straws, frozen in liquid nitrogen vapour, and stored in liquid nitrogen. Samples were thawed at 37°C for 30 s. Post-thaw sperm quality was evaluated together with oxidative stress markers, including total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI).
The 2% CLP group showed the highest post-thaw sperm quality, whereas the lowest performance was observed in the 4% CLP group. Additionally, the 2% CLP group exhibited a more favourable oxidative profile, with higher TAS and lower TOS and OSI values than all other groups.
CLP supplementation exerted dose-dependent effects on sperm quality and oxidative balance in canine semen cryopreservation. A 2% CLP concentration appears to be the most effective for improving post-thaw sperm quality.
PMID:
42554047
Bibliographic data and abstract were imported from PubMed on 05 Aug 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 3
- Comments 0