Authors
Lejing Yao, Xiaoming Sun, Cheng-Yu Li
Published in
Analytica chimica acta. Volume 1418. Pages 345880. Oct 08, 2026. Epub Jun 24, 2026.
Abstract
DNA nanoamplifiers constructed by isothermal protease-free nucleic acid amplifications are promising for fluorescence imaging of disease biomarkers in live biosystems. However, they are still hindered by cumbersome cascade architectures, suboptimal sensitivity, and compromised analytical precision.
Herein, we devise a monolithic triple auto-enhanced DNA nanoamplifier under orthogonal dual-response of direct-lock near-infrared (NIR) photocage and endogenous apurinic/apyrimidinic endonuclease 1 (APE1) gate. The nanoamplifier incorporates three consecutive amplification processes: analyte-recycling amplification, self-propagating Mn2+-reliant DNAzyme-mediated hybridization chain reaction (HCR), and DNAzyme reassembly-driven iterative HCR. Orthogonal dual-response is performed through an initial direct-lock NIR photocage enabled by ultraviolet-emitting upconversion luminescence and photolytic moieties directly tethered to apurinic/apyrimidinic sites, followed by an endogenous APE1 gate. Using malignant tumor-associated microRNA-155 for conceptual verification, the method exhibits ultrasensitive (limit of detection down to 5.31 fM) and highly specific biosensing performance. Moreover, it allows high-precision fluorescence imaging of low-abundance analytes in live cells and animal models.
This work propels the advancement of DNA nanoamplifiers and offers a potent approach for bioassay applications.
PMID:
42567614
Bibliographic data and abstract were imported from PubMed on 08 Aug 2026.
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