Lipopolysaccharide-Specific Aptasensor: A Spectroscopic Approach for Identifying Gram-Negative Bacteria.

Authors

Hamidreza Majidifard, Rahman Emamzadeh, Donald Keith Roper, Mahboobeh Nazari

Published in

Analytical science advances. Volume 7. Issue 2. Pages e70104. Epub Aug 06, 2026.

Abstract

Differentiating bacteria into Gram-negative and Gram-positive categories is a standard method of identification in biological science and is essential for understanding bacterial characteristics. This study presents a novel aptasensor that integrates an anti-lipopolysaccharide (LPS) aptamer with a G-quadruplex-based DNAzyme. This aptasensor enables spectroscopy-based detection of LPS-containing Gram-negative bacteria. With a detection limit of approximately 2.7 × 10⁵ cells/mL for Escherichia coli, this aptasensor offers an efficient and straightforward solution for laboratory detection of Gram-negative bacteria.

PMID:
42568754
Bibliographic data and abstract were imported from PubMed on 08 Aug 2026.

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