A Rare Case of a Newborn with Blocked RhD Antigen Caused by Low-Titer Antibody.

Authors

Ling Li, Se Zhen, Danzeng Bianjue, Xueni Chen

Published in

Clinical laboratory. Volume 72. Issue 8. Aug 01, 2026.

Abstract

Hemolytic disease of the fetus and newborn (HDFN) is a condition characterized by severe hemolysis and anemia in fetuses or neonates. To our knowledge, this is the first reported case of a newborn with blocked RhD antigen caused by low-titer antibody.
Peripheral blood samples were collected from the newborn upon admission. ABO and RhD blood typing was performed using a microcolumn gel agglutination test. Direct antiglobulin test (DAT) was performed using polyspecific anti-human globulin. Indirect antiglobulin test (IAT) was used to screen for irregular antibodies in the serum. Thermal elution was carried out by incubating the newborn's red blood cells at 56℃ for 10 minutes, and the resulting eluate was tested for non-ABO antibodies and their titers by IAT.
At birth, the infant was typed as O Rh-positive. However, pre-transfusion retesting indicated O Rh-negative status. The DAT was negative, whereas the IAT was positive (2+). Subsequent ABO-compatible elution testing also yielded a positive result (1+). The antibody titer was measured at 1:16.
This is the first reported case of blocked RhD antigen caused by low-titer antibody. This case high-lights the critical importance of comprehensive serological investigation in affected neonates to guide appropriate clinical management and transfusion strategy.

PMID:
42570654
Bibliographic data and abstract were imported from PubMed on 09 Aug 2026.

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