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Enhanced Activity of Eugenol Pickering Emulsion Using Lactoferrin-Chlorogenic Acid Complex as a Green Stabilizer.

Created on 09 Aug 2026

Authors

Xiyao Dong, Tongyan Liu, Mengfan Li, Xinying Wang, Qingxiang Guan

Published in

Chemistry & biodiversity. Volume 23. Issue 8. Pages e71588.

Abstract

Exploiting natural and bioactive stabilizers to fabricate green emulsions has attracted increasing attention. In this work, lactoferrin (LF) was combined with chlorogenic acid (CGA) to construct the LF-CGA complex to prepare a eugenol (EG) Pickering emulsion (ELC/PE). The surface activity and binding mechanism of LF-CGA were explored. Additionally, the antioxidant, antibacterial, and anti-inflammatory capacities of ELC/PE were compared. The contact angle of LF-CGA approached 90° (81.9°), indicating enhanced stability at the oil-water interface. The interaction between LF and CGA was primarily driven by static quenching and hydrogen bonding. The ELC/PE consisted of 1.5 wt% LF-CGA, with distilled water and EG as the aqueous and oil phases (15:1 v/v, aqueous/oil). The DPPH-, O2 -, and ABTS+ scavenging rates of ELC/PE were 86.3%, 75.3%, and 83.4%, respectively. The ELC/PE displayed superior antibacterial ability, along with inhibition rates of 99.0% and 99.5% against Candida albicans and Staphylococcus aureus, respectively. Additionally, the ELC/PE exhibited the most significant suppression of ear swelling (81.8%), confirming its anti-inflammatory ability. These values were the highest among all experimental groups. The LF-CGA complex acts as an emulsifier to enhance EG bioactivities. This novel complex holds great potential for expanding the practical applications of poorly soluble bioactive compounds.

PMID:
42571634
Bibliographic data and abstract were imported from PubMed on 09 Aug 2026.

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