AuNP decorated-Ni-MOF nanosheet based cDNA sensor for detection of miRNA-128 in Alzheimer's disease.

Authors

Sonia Rani, Karthik Vinay Mahesh, Rajat Sandhir, Nirmal Prabhakar

Published in

Talanta. Volume 312. Issue Pt A. Pages 130405. Aug 05, 2026. Epub Aug 05, 2026.

Abstract

A cDNA sensor based on gold nanoparticles (Au) supported on nickel metal-organic framework nanosheets (MOF-NS) modified fluorine-tin oxide (FTO) glass electrode for the ultrasensitive and selective detection of miRNA-128 biomarker associated with the progression of Alzheimer's disease (AD) has been reported. The MOF-NS and Au nanoparticles were electrodeposited onto FTO electrode using chronoamperometry to immobilize 5'-biotinylated DNA (btn-cDNA) complementary to miRNA-128 by streptavidin-biotin interaction, generating the modified electrode (btn-cDNA/SV/Au/MOF-NS/FTO). The fabricated electrode was characterized using various surface characterization techniques, including FESEM, XPS, FTIR, and electrochemical methods. The fabricated electrode was then utilized for the selective detection of miRNA-128 using electrochemical impedance spectroscopy. The results indicated a linear response range of 1.0 × 10^-1 fM-1.0 × 10² nM with limit of detection of 0.017 fM, and sensitivity of 180.11 Ω fM^-1cm^-2. Moreover, the cDNA sensor showed satisfactory performance with real serum samples from AD patients and healthy individuals, as validated by real time-PCR technique with area under curve of 0.93 and a sensitivity of 90%, respectively, demonstrating the potential of present cDNA sensing approach in the biomedical field.

PMID:
42571753
Bibliographic data and abstract were imported from PubMed on 10 Aug 2026.

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