Authors
Yasemin Erdoğan, Ediz Kağan Özgen, Murat Özmen, Elif Karadeniz Pütür, Nebahat Polat, Ersan Pütür, Mücahit Harmancı, Ercan Atalay, Ahmet Temur, Meryem Güloğlu, Lütfullah Celep, Seyda Cengiz, Mehmet Cemal Adıgüzel, Cihan Öz, Timur Gülhan, Alper Çiftci, Erhan Çiftgül
Published in
Veterinary medicine and science. Volume 12. Issue 5. Pages e71151.
Abstract
Escherichia coli is the most commonly isolated bacterium in cases of diarrhoea in calves under the age of 2 months.
This study aimed to identify E. coli strains with immunogenic potential by characterizing isolates from diarrheic calves through antigenic profiling and genotyping.
The phenotypic and genotypic antibiotic resistance characteristics and virulence genes of the E. coli strains isolated from 500 faecal samples were examined, of which 88 were further genotyped using the Multiple-Locus Variable-Number Tandem Repeat Analysis (MLVA) method. Subsequently, 20 E. coli strains were selected based on their antibiotic resistance patterns and genetic typing (MLVA) results, and subjected to an analysis of their outer membrane vesicle profile.
Among these, strains Ec048 and Ec026 exhibited high OMV diversity and were identified as promising candidates for further immunogenic evaluation. Immunization of experimental animals with these strains enhanced humoural immune responses (IgG, IgM, CD20), whereas cellular immune parameters (CD4, CD8) showed no significant change.
In conclusion, the study demonstrates that strains Ec048 and Ec026 elicit strong humoural immune responses, indicating their potential for vaccine development. However, protective efficacy was not assessed, and further studies including challenge experiments are required to confirm their suitability as vaccine strains.
PMID:
42571708
Bibliographic data and abstract were imported from PubMed on 10 Aug 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 11
- Comments 0