Compositional and Functional Characterization of Glycolipid Biosurfactants from an Olive-Derived Lactiplantibacillus plantarum.

Authors

Jong Woo Hyeon, Min Hui Jeon, Jun-Tae Bae, Man-Young Jung

Published in

Journal of microbiology and biotechnology. Volume 36. Pages e2606027. Aug 10, 2026. Epub Aug 10, 2026.

Abstract

Biosurfactants from lactic acid bacteria are attractive as safe, multifunctional ingredients for food and cosmetic applications, yet many remain only partially characterized. An olive-derived strain was identified as Lactiplantibacillus plantarum J2K-1229 by 16S rRNA gene and genome-based phylogeny. Grown in rapeseed oil-containing medium, it secreted extracellular material with surface/interfacial activity, and its cell-free supernatant emulsified diverse plant oils (emulsification index after 24 h, 26.67-41.33%). A purified biosurfactant fraction gave carbohydrate- and lipid-positive, ninhydrin-negative thin-layer chromatography and Fourier transform infrared bands of a hydroxylated, ester- and carboxylic acid-containing lipid. Gas chromatography, NMR, and HPAEC-PAD indicated a complex, lipid-dominant mixture with major oleic and palmitic acids, NMR signals consistent with co-extracted phenyllactic acid-type metabolites, and fructose as the only detected monosaccharide. Genome mining revealed glucosyltransferases for glucose-based glycolipids but no fructosyltransferase, suggesting that the detected fructose may represent co-extracted free fructose, possibly released as a glucansucrase by-product. The preparation inhibited Candida albicans in disk diffusion assays and was non-cytotoxic to RAW 264.7 macrophages and HaCaT keratinocytes up to 200 ppm. In lipopolysaccharide-stimulated macrophages it suppressed nitric oxide production (88.07% at 200 ppm) and inducible nitric oxide synthase expression, and in cytokine-stimulated keratinocytes it reduced TARC, MDC, and RANTES expression. Overall, this olive-derived L. plantarum produces a multicomponent, glycolipid-containing biosurfactant preparation with emulsifying, antifungal, and anti-inflammatory activities suited to cosmetic and personal-care applications.

PMID:
42572799
Bibliographic data and abstract were imported from PubMed on 10 Aug 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.