Observation of glutathione intra- and extracellular membrane with large Stokes shift probe modified by long alkyl chains.

Authors

Lin Li, Shao-Bin Sun, Dan Qiao, Jing Zhou, Lin-Qing Wang, Jian-Yong Wang

Published in

Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology. Aug 11, 2026. Epub Aug 11, 2026.

Abstract

A naphthylimide dye-based fluorescent probe (Nap-Mem-GSH) was synthesized for the detection of Glutathione inside and outside cell membranes. This probe carried a 2-Hydroxyquinoline fragment as a recognition site on top of a naphthalimide dye, which exhibits better selective and fluorimetric response toward Glutathione in natural media. The long alkyl chain confers a cell membrane targeting role to Nap-Mem-GSH. Therefore, the detection of intra-and extracellular glutathione inside and outside the cell membrane can be realized by Nap-Mem-GSH. Meanwhile, Nap-Mem-GSH not only has a large Stokes shift (160 nm), a low detection limit and a rapid response toward GSH, but also has the advantages of low cytotoxicity and good membrane permeability to living cells, and has been successfully applied to effectively detect and image intracellular glutathione by confocal fluorescence imaging.

PMID:
42579264
Bibliographic data and abstract were imported from PubMed on 11 Aug 2026.

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