Authors
Tram Thi Thu Nguyen, Na-Young Choi, Min-Ha Kim, Hyunmo Choi, Dong-Gwan Kim, Young-Im Choi, Jae-Heung Ko
Published in
Plant cell reports. Volume 45. Issue 9. Aug 11, 2026. Epub Aug 11, 2026.
Abstract
When ectopically expressed in hybrid poplar, the gymnosperm protein PdeLAZY1 confers LAZY1-like activity, whereas deletion of Domain V abolishes this activity without conferring TAC1-like function. The IGT gene family regulates plant architecture by controlling lateral organ angles. TAC1 is proposed to have evolved from an ancestral LAZY-like gene through loss of the conserved C-terminal Domain V, but this has not been tested functionally. We expressed PdeLAZY1, a LAZY1 homolog from the gymnosperm Pinus densiflora, and its Domain V-deleted variant (PdeLAZY1ΔV) under the 35S promoter in wild-type hybrid poplar (clone BH) and in a CRISPR-generated tac1 mutant. In wild-type poplar, PdeLAZY1 significantly reduced petiole angle, showing that a gymnosperm LAZY1 is competent to promote upright growth in an angiosperm context, whereas PdeLAZY1ΔV significantly increased it, indicating that Domain V is required for this activity. In the tac1 background, however, neither construct significantly increased petiole angle relative to the untransformed control, so PdeLAZY1ΔV did not complement loss of TAC1. Domain V is therefore required for the LAZY1-type activity of PdeLAZY1, but its loss alone is not sufficient to confer TAC1-like function in poplar. Because these are ectopic overexpression experiments, they test competence rather than endogenous function; within this limit, the data are consistent with a stepwise model in which changes beyond Domain V loss were required for TAC1 evolution.
PMID:
42579158
Bibliographic data and abstract were imported from PubMed on 11 Aug 2026.
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